SYTL2 ELISA Kit (Human) (OKCD01020)

Catalog No: OKCD01020 (Formerly GWB-KBBRC7)

Size:96WELLS

Human SYTL2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptotagmin Like Protein 2 (SYTL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptotagmin Like Protein 2 (SYTL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptotagmin Like Protein 2 (SYTL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Synaptotagmin Like Protein 2 (SYTL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptotagmin Like Protein 2 (SYTL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptotagmin Like Protein 2 (SYTL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SYTL2 Microplate: 96 Wells (12 x 8 Well Strips)
  • SYTL2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated SYTL2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Synaptotagmin Like Protein 2 (SYTL2). No significant cross-reactivity or interference between Synaptotagmin Like Protein 2 (SYTL2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SYTL2
• Gene Full Name: synaptotagmin like 2
• Alias Symbols: breast cancer-associated antigen SGA-72M;CHR11SYT;chromosome 11 synaptotagmin;EXO4;exophilin-4;PPP1R151;protein phosphatase 1, regulatory subunit 151;SGA72M;SLP2;SLP2A;synaptotagmin-like protein 2.
• NCBI Gene Id: 54843
• Protein Name: Synaptotagmin-like protein 2
• Description of Target: Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol-4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon-containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca(2+) while binding to PIP2 is Ca(2+) insensitive.
• Uniprot ID: Q9HCH5
• Protein Accession #: NP_001156423
• Nucleotide Accession #: NM_001162951.2
• Protein Size (# AA): 934