Catalog No: OKCD00484 (Formerly GWB-KBBIP1)
Size:96WELLS
Price: $675.00
SKU
OKCD00484
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Datasheets/ManualsPrintable datasheet for SYNE2 ELISA Kit (Human) (OKCD00484)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nesprin 2 (Nesp2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nesprin 2 (Nesp2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nesprin 2 (Nesp2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nesprin 2 (Nesp2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesprin 2 (Nesp2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesprin 2 (Nesp2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SYNE2 Microplate96 Wells (12 x 8 Well Strips)
SYNE2 Lyophilized Standard2 x 10 ng
100X Biotinylated SYNE2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nesprin 2 (Nesp2). No significant cross-reactivity or interference between Nesprin 2 (Nesp2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSYNE2
Gene Full Namespectrin repeat containing nuclear envelope protein 2
Alias SymbolsEDMD5;KASH domain-containing protein 2;KASH2;Nesp2;nesprin 2;nesprin-2;NUA;NUANCE;nuclear envelope spectrin repeat protein 2;nucleus and actin connecting element protein;polytrophin;spectrin repeat containing, nuclear envelope 2;synaptic nuclear envelope protein 2;synaptic nuclei expressed gene 2;SYNE-2;TROPH.
NCBI Gene Id23224
Protein NameNesprin-2
Description of TargetMulti-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 may act redundantly. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis.
Uniprot IDQ8WXH0
Protein Accession #NP_055995
Nucleotide Accession #NM_015180.4
Protein Size (# AA)6885
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