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Catalog No: OKCD02914
Size:96WELLS
Price: $700.00
SKU
OKCD02914
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Datasheets/ManualsPrintable datasheet for SYN1 ELISA Kit (Mouse) (OKCD02914)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synapsin I (SYN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synapsin I (SYN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synapsin I (SYN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Synapsin I (SYN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synapsin I (SYN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synapsin I (SYN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SYN1 Microplate96 Wells (12 x 8 Well strips)
SYN1 Lyophilized Standard2
100X Biotinylated SYN1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample Typetissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Sensitivity0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Synapsin I (SYN1). No significant cross-reactivity or interference between Synapsin I (SYN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSyn1
Gene Full Namesynapsin I
Alias SymbolsSyn;Syn-1;Syn1-S;Synapsin I;synapsin-1;truncated synapsin 1-S.
NCBI Gene Id20964
Protein NameSynapsin-1
Description of TargetNeuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.
Uniprot IDO88935
Protein Accession #NP_001104250.1
Nucleotide Accession #NM_001110780.1
Protein Size (# AA)706
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