Syk Antibody (Phospho-Tyr525/526) (OAAB16125)

Catalog No: OAAB16125

Size:200ul

Western blot analysis of lysates from Raji cell line, untreated or treated with TPA, 200nM, 30min, using (Cat. #OAAB16125)(upper) or Tubulin (lower).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC= breast carcinoma; HC= hepatocarcinoma

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat, Pig
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Cellular Location: Cell membrane. Cytoplasm, cytosol.
  Tissue Location: Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro- and pre-B cells to plasma cells.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification
• Concentration: 0.35 mg/ml (lot specific)
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:250
  IHC-P: 1:50~100
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: SYK
• Alias Symbols: p72-Syk
• NCBI Gene Id: 6850
• Protein Name: Tyrosine-protein kinase SYK
• Description of Target: Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.
• Uniprot ID: P43405
• Protein Accession #: NP_001128524.1
• Molecular Weight: 72 kDa