sVEGFR1/sFLT1 ELISA Kit (Human) (OKBB00274)

Catalog No: OKBB00274 (Formerly GWB-ZZD096)

Size:96 Tests

Human FLT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human sVEGFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sVEGFR1 has been precoated onto 96-well plates. Standards(NS0, S27-H687) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sVEGFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human sVEGFR1 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	367	2835	5213	530	3015	6142
  St. Dev.	15.41	130.4	255.4	27.56	180.9	399.2
  %CV	4.19	4.59	4.89	5.2	6	6.49

• ELISA Kit Component:
  • Lyophilized recombinant human FLT1 standard: 10 ng/tube x 2
  • Anti-human FLT1 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human FLT1 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 156pg/ml-10, 000pg/ml
  Principle: Aviva’s human sVEGFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human sVEGFR1 specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sVEGFR1 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (EDTA)
• Sensitivity: <30 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Imbert, A.; Rosnet, O.; Marchetto, S.; Ollendorff, V.; Birnbaum, D.; Pebusque, M.-J. : Characterization of a yeast
  artificial chromosome from human chromosome band 13q12 containing the FLT1 and FLT3 receptor-type tyrosine
  kinase genes. Cytogenet. Cell Genet. 67: 175-177, 1994.
  2. Niida, S.; Kondo, T.; Hiratsuka, S.; Hayashi, S.-I.; Amizuka, N.; Noda, T.; Ikeda, K.; Shibuya, M. : VEGF receptor 1
  signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient
  mice. Proc. Nat. Acad. Sci. 102: 14016-14021, 2005.
• Specificity: Natural and recombinant human Svegfr1
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S27-H687
• Description: For quantitative detection of human sVEGFR1 in cell culture supernatants, serum and plasma(EDTA).

Target Info

• Gene Symbol: FLT1
• Gene Full Name: fms-related tyrosine kinase 1
• Alias Symbols: FLT 1, FLT1, Flt-1, sflt 1, sFLT1, Soluble VEGFR1 variant 2, Soluble VEGFR1 variant 21, sVEGFR1, Tyrosine protein kinase FRT, Tyrosine-protein kinase FRT, VEGF Receptor 1, VEGFR 1, VEGFR-1, VGFR1
• NCBI Gene Id: 2321
• Protein Name: Vascular endothelial growth factor receptor 1
• Description of Target: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
• Uniprot ID: P17948
• Protein Accession #: NP_001153392.1
• Nucleotide Accession #: NM_001159920.1