SUMO2 Antibody - C-terminal region (OAAB09116)

Catalog No: OAAB09116

Size:400ul

Western blot analysis of SUMO2 (arrow) using rabbit polyclonal SUMO2 Antibody (Cat.#OAAB09116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2 gene.

SUMO2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

The SUMO2 C-term Antibody (Cat.#OAAB09116) is used in Western blot to detect SUMO2 in GST-SUMO2 fusion protein (lane 1) and HL60 cell lysate (lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Pig, Zebrafish
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC, WB
• Additional Information: Precautions: SUMO2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins
  Cellular Location: Nucleus. Note=Nuclear bodies.
  Tissue Location: Broadly expressed.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SUMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-93 amino acids from the C-terminal region of human SUMO2.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Pig; Rat; Zebrafish
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  SUMO2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: SUMO2
• Alias Symbols: HSMT3, SMT3B, SUMO3, Smt3A, SMT3H2
• NCBI Gene Id: 6613
• Protein Name: Small ubiquitin-related modifier 2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P61956
• Protein Accession #: NP_001005849.1
• Molecular Weight: 10871 Da