Sumo1 Antibody (OAAF01967)

Catalog No: OAAF01967 (Formerly GWB-ASD236)

Size:100 ug

Western blot analysis of extracts from 293 cells, using Sumo1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Sumo1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using Sumo1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Sumo1.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: Sumo1 antibody detects endogenous levels of total SUMO-1 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000
  IHC: 50~100
  IF: 1:100~1:500
  ELISA: 1:20000
• Reference: Margarita Vigodner, Am J Physiol Endocrinol Metab, May 2006; 290: E1022 - E1033.
  Adrian Minty, J. Biol. Chem., Nov 2000; 275: 36316.
  Deborah A. Sampson, J. Biol. Chem., Jun 2001; 276: 21664 - 21669.
  Hidehisa Takahashi, J. Biol. Chem., Feb 2

Target Info

• Gene Symbol: SUMO1
• Gene Full Name: small ubiquitin like modifier 1
• Alias Symbols: DAP1;GAP modifying protein 1;GAP-modifying protein 1;GMP1;OFC10;PIC1;SENP2;sentrin;small ubiquitin-related modifier 1;SMT3;SMT3 homolog 3;SMT3 suppressor of mif two 3 homolog 1;SMT3C;SMT3H3;ubiquitin-homology domain protein PIC1;ubiquitin-like protein SMT3C;ubiquitin-like protein UBL1;UBL1.
• NCBI Gene Id: 7341
• Protein Name: Small ubiquitin-related modifier 1
• Description of Target: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376).
• Uniprot ID: P63165
• Protein Accession #: NP_001005781.1
• Molecular Weight: 11 kDa