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Catalog No: OKCD02830
Size:96WELLS
Price: $650.00
SKU
OKCD02830
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Datasheets/ManualsPrintable datasheet for SULT1A1 ELISA Kit (Mouse) (OKCD02830)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfotransferase 1A1 (SULT1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sulfotransferase 1A1 (SULT1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sulfotransferase 1A1 (SULT1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sulfotransferase 1A1 (SULT1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SULT1A1 Microplate96 Wells (12 x 8 Well strips)
SULT1A1 Lyophilized Standard2
100X Biotinylated SULT1A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phenol Sulfotransferase (PST). No significant cross-reactivity or interference between Phenol Sulfotransferase (PST) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSULT1A1
Gene Full Namesulfotransferase family 1A, phenol-preferring, member 1
Alias SymbolsAryl sulfotransferase;Phenol sulfotransferase;Phenol/aryl sulfotransferase;ST1A4;Sulfokinase.
Protein NameSulfotransferase 1A1
Description of TargetSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk (By similarity).
Uniprot IDP52840
Protein Accession #NP_598431.1
Nucleotide Accession #NM_133670.1
Protein Size (# AA)291
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