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Catalog No: OKEH03277
Size:96T
Price: $525.00
SKU
OKEH03277
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology STUB1 ELISA Kit (Human) (OKEH03277) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STUB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for STUB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STUB1 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.4% (n = 20)
Mean Inter-assay CV%: <= 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-STUB1 Microplate96 Wells (12 x 8 Well strips)
STUB1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated STUB1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Human E3 ubiquitin-protein ligase CHIP
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSTUB1
Gene Full NameSTIP1 homology and U-box containing protein 1
Alias Symbolsantigen NY-CO-7;carboxy terminus of Hsp70-interacting protein;CHIP;CLL-associated antigen KW-8;E3 ubiquitin-protein ligase CHIP;heat shock protein A binding protein 2 (c-terminal);HSPABP2;NY-CO-7;RING-type E3 ubiquitin transferase CHIP;SCA48;SCAR16;SDCCAG7;serologically defined colon cancer antigen 7;STIP1 homology and U box-containing protein 1;STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase;UBOX1.
NCBI Gene Id10273
Protein NameQ9UNE7
Description of TargetE3 ubiquitin-protein ligase CHIP
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UNE7
Protein Accession #NP_001280126.1
Nucleotide Accession #NM_001293197.1
Protein Size (# AA)303
Molecular Weight35 kDa
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