STAT4 ELISA Kit (Human) (OKCD02897)

Catalog No: OKCD02897

Size:96WELLS

Human STAT4 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human|Mouse|Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 4 (STAT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 4 (STAT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 4 (STAT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Signal Transducer And Activator Of Transcription 4 (STAT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 4 (STAT4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 4 (STAT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-STAT4 Microplate: 96 Wells (12 x 8 Well strips)
  • STAT4 Lyophilized Standard: 2
  • 100X Biotinylated STAT4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.108 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 4 (STAT4). No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 4 (STAT4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: STAT4
• Gene Full Name: signal transducer and activator of transcription 4
• Alias Symbols: signal transducer and activator of transcription 4;signal transducer and activator of transcription 4 variant 3;SLEB11.
• NCBI Gene Id: 6775
• Protein Name: Signal transducer and activator of transcription 4
• Description of Target: Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
• Uniprot ID: Q14765
• Protein Accession #: NP_001230764.1
• Nucleotide Accession #: NM_001243835.1
• Protein Size (# AA): 748