Catalog No: OKCD02896
Size:96WELLS
Price: $650.00
SKU
OKCD02896
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for STAT1 ELISA Kit (Human) (OKCD02896) |
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Predicted Species Reactivity | Homo sapiens|Human|Mouse|Rat | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 1 (STAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Signal Transducer And Activator Of Transcription 1 (STAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.115 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 1 (STAT1). No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 1 (STAT1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | STAT1 |
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Gene Full Name | signal transducer and activator of transcription 1 |
Alias Symbols | CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;signal transducer and activator of transcription 1, 91kD;signal transducer and activator of transcription 1, 91kDa;signal transducer and activator of transcription 1-alpha/beta;STAT91;transcription factor ISGF-3 components p91/p84. |
NCBI Gene Id | 6772 |
Protein Name | Signal transducer and activator of transcription 1-alpha/beta |
Description of Target | Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. |
Uniprot ID | P42224 |
Protein Accession # | NP_009330.1 |
Nucleotide Accession # | NM_007315.3 |
Protein Size (# AA) | 750 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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