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Catalog No: OKEH03717
Size:96T
Price: $625.00
SKU
OKEH03717
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology STAR ELISA Kit (Rat) (OKEH03717) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Star has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Star is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Star captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Star Microplate96 Wells (12 x 8 Well strips)
Star Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Star Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.182 ng/mL
SpecificityNatural and recombinant Rat Steroidogenic acute regulatory protein, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolStar
Gene Full Namesteroidogenic acute regulatory protein
Alias SymbolsstARD1;START domain-containing protein 1;steroidogenic acute regulatory protein, mitochondrial.
NCBI Gene Id25557
Protein NameP97826
Description of TargetSteroidogenic acute regulatory protein, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P97826
Protein Accession #NP_113746.1
Nucleotide Accession #NM_031558.3
Protein Size (# AA)284
Molecular Weight32 kDa
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