STAR ELISA Kit (Human) (OKCD00508)

Catalog No: OKCD00508 (Formerly GWB-KBBPK9)

Size:96WELLS

Human STAR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroidogenic Acute Regulatory Protein (STAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroidogenic Acute Regulatory Protein (STAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroidogenic Acute Regulatory Protein (STAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Steroidogenic Acute Regulatory Protein (STAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroidogenic Acute Regulatory Protein (STAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroidogenic Acute Regulatory Protein (STAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-STAR Microplate: 96 Wells (12 x 8 Well Strips)
  • STAR Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated STAR Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Steroidogenic Acute Regulatory Protein (STAR). No significant cross-reactivity or interference between Steroidogenic Acute Regulatory Protein (STAR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: STAR
• Gene Full Name: steroidogenic acute regulatory protein
• Alias Symbols: cholesterol trafficker;mitochondrial steroid acute regulatory protein;StAR related lipid transfer (START) domain containing 1;STARD1;START domain containing 1;START domain-containing protein 1;steroid acute regulatory protein;steroidogenic acute regulator;steroidogenic acute regulatory protein, mitochondrial;testis secretory sperm-binding protein Li 241mP.
• NCBI Gene Id: 6770
• Protein Name: Steroidogenic acute regulatory protein, mitochondrial
• Description of Target: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
• Uniprot ID: P49675
• Protein Accession #: NP_000340
• Nucleotide Accession #: NM_000349.2
• Protein Size (# AA): 285