Srebf2 ELISA Kit (Rat) (OKEH04663)

Catalog No: OKEH04663

Size:96T

Rat Srebf2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Srebf2 ELISA Kit (Rat) (OKEH04663) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Srebf2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Srebf2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Srebf2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Srebf2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Srebf2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Srebf2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Rat Sterol regulatory element-binding protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Srebf2
• Gene Full Name: sterol regulatory element binding transcription factor 2
• Alias Symbols: Srebf2_retired;SREBP2;SREBP-2;sterol regulatory element binding factor 2;sterol regulatory element-binding protein 2;Sterol regulatory element-binding transcription factor 2.
• NCBI Gene Id: 300095
• Protein Name: Q3T1I5
• Description of Target: Sterol regulatory element-binding protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q3T1I5
• Protein Accession #: NP_001028866.1
• Nucleotide Accession #: NM_001033694.1
• Protein Size (# AA): 1133
• Molecular Weight: 123 KDa