Catalog No: OKCD02894
Size:96WELLS
Price: $800.00
SKU
OKCD02894
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Datasheets/ManualsPrintable datasheet for SRD5A1 ELISA Kit (Rat) (OKCD02894)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 1 (SRD5a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 1 (SRD5a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 1 (SRD5a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Steroid 5 Alpha Reductase 1 (SRD5a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 1 (SRD5a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 1 (SRD5a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SRD5A1 Microplate96 Wells (12 x 8 Well strips)
SRD5A1 Lyophilized Standard2
100X Biotinylated SRD5A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 1 (SRD5a1). No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 1 (SRD5a1) and analogues was observed
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSrd5a1
Gene Full Namesteroid 5 alpha-reductase 1
Alias Symbols3-oxo-5-alpha-steroid 4-dehydrogenase 1;S5AR 1;SR type 1;Steroid 5-alpha-reductase 1;steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1).
NCBI Gene Id24950
Protein Name3-oxo-5-alpha-steroid 4-dehydrogenase 1
Description of TargetConverts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Uniprot IDP24008
Protein Accession #NP_058766.2
Nucleotide Accession #NM_017070.3
Protein Size (# AA)259
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