SPTLC3 ELISA Kit (Human) (OKCD00522)

Catalog No: OKCD00522 (Formerly GWB-KBBDP6)

Size:96WELLS

Human SPTLC3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SPTLC3 Microplate: 96 Wells (12 x 8 Well Strips)
  • SPTLC3 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SPTLC3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SPTLC3
• Gene Full Name: serine palmitoyltransferase long chain base subunit 3
• Alias Symbols: C20orf38;dJ718P11;dJ718P11.1;hLCB2b;LCB 3;LCB2B;LCB3;long chain base biosynthesis protein 2b;long chain base biosynthesis protein 3;serine palmitoyltransferase 3;serine palmitoyltransferase, long chain base subunit 2-like (aminotransferase 2);serine-palmitoyl-CoA transferase 3;SPT 3;SPT3;SPTLC2L.
• NCBI Gene Id: 55304
• Protein Name: Serine palmitoyltransferase 3
• Description of Target: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.
• Uniprot ID: Q9NUV7
• Protein Accession #: NP_060797
• Nucleotide Accession #: NM_018327.2
• Protein Size (# AA): 552