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Catalog No: OKCD00466 (Formerly GWB-KBBVZ7)
Size:96WELLS
Price: $725.00
SKU
OKCD00466
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Datasheets/ManualsPrintable datasheet for SPON2 ELISA Kit (Rat) (OKCD00466)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)99-105%80-97%88-95%90-104%
EDTA plasma(n=5)91-98%90-97%94-105%91-101%
heparin plasma(n=5)93-105%98-105%87-97%91-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2 (SPON2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 2 (SPON2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 2 (SPON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Spondin 2 (SPON2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)87-9692
EDTA plasma(n=5)81-9388
heparin plasma(n=5)78-9187
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spondin 2 (SPON2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spondin 2 (SPON2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Spon2 Microplate96 Wells (12 x 8 Well Strips)
Spon2 Lyophilized Standard2 x 10 ng
100X Biotinylated Spon2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.068 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Spondin 2 (SPON2). No significant cross-reactivity or interference between Spondin 2 (SPON2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLOC100910790|Spon2
Gene Full Namespondin 2|spondin-2-like
Alias SymbolsF-spondin;mindin;spondin 2, extracellular matrix protein;spondin-2;spondin-2-like.
NCBI Gene Id100910790|171569
Protein NameSpondin-2
Description of TargetCell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity).
Uniprot IDQ9WV75
Protein Accession #NP_612542
Nucleotide Accession #NM_138533.3
Protein Size (# AA)330
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