Catalog No: OKEH04577
Size:96T
Price: $525.00
SKU
OKEH04577
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SPINK1 ELISA Kit (Human) (OKEH04577) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPINK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SPINK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPINK1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 102% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay Precision - < 4.8%, n = 20 Mean Inter-Assay Precision - < 8.0%, n = 20 | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.13 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Serine protease inhibitor Kazal-type 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | SPINK1 |
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Gene Full Name | serine peptidase inhibitor Kazal type 1 |
Alias Symbols | pancreatic secretory trypsin inhibitor;PCTT;PSTI;serine protease inhibitor Kazal-type 1;serine protease inhibitor, Kazal type 1;Spink3;TATI;TCP;tumor-associated trypsin inhibitor. |
NCBI Gene Id | 6690 |
Protein Name | P00995 |
Description of Target | Serine protease inhibitor Kazal-type 1 |
Uniprot ID | https://www.uniprot.org/uniprot/P00995 |
Protein Accession # | NP_003113.2 |
Nucleotide Accession # | NM_003122.4 |
Protein Size (# AA) | 79 |
Molecular Weight | 9 KDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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