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Catalog No: OKEH01094
Size:96T
Price: $525.00
SKU
OKEH01094
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SPARCL1 ELISA Kit (Mouse) (OKEH01094) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse SPARCL1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SPARCL1 and incubated. The SPARCL1 found in the sample or standards competes with the biotinylated SPARCL1 for limited binding sites on the immobilized anti-mouse SPARCL1 antibody. Excess unbound biotinylated SPARCL1 and sample or standard SPARCL1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SPARCL1 captured in the well and inversely proportional to the amount of SPARCL1 which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-SPARCL1 Microplate96 Wells (12 x 8 Well strips)
SPARCL1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X SPARCL1-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.397 ng/mL
SpecificityNatural and recombinant Mouse SPARC-like protein 1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolSparcl1
Gene Full NameSPARC-like 1
Alias SymbolsEcm2;extracellular matrix protein 2;he;hevin;mas;mast9;matrix glycoprotein Sc1;Sc;Sc1;SPARC-like 1 (mast9, hevin);SPARC-like protein 1.
NCBI Gene Id13602
Protein NameP70663
Description of TargetSPARC-like protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P70663
Protein Accession #NP_034227.3
Nucleotide Accession #NM_010097.4
Protein Size (# AA)650
Molecular Weight72 kDa
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