SPARCL1 ELISA Kit (Human) (OKBB00659)

Catalog No: OKBB00659

Size:96 Wells

Human SPARCL1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human SPARCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been precoated onto 96-well plates. Standards(NS0, I17-F664) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human SPARCL1 amount of sample captured in plate.
• ELISA Kit Range: 46.9 pg/ml - 3,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	269	1195	2030	321	1254	2208
  SD	11.03	57.36	105.6	18.3	79	167.8
  CV%	4.1	4.8	5.2	5.7	6.3	7.6

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human SPARCL1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human SPARCL1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human SPARCL1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants and serum
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human SPARCL1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: I17-F664
• Description: For quantitative detection of human SPARCL1 in cell culture supernatants and serum.

Target Info

• Gene Symbol: SPARCL1
• Gene Full Name: SPARC-like 1 (hevin)
• Alias Symbols: Ecm2, Extracellular matrix protein 2, Hevin, High endothelial venule protein, MAST 9, mast9, Matrix glycoprotein Sc1, PIG33, proliferation inducing protein 33, RAGS1 antigen, SC1, SPARC like 1, SPARC-like protein 1, Sparcl1, SPRL1_HUMAN
• NCBI Gene Id: 8404
• Protein Name: SPARC-like protein 1
• Description of Target: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.
• Uniprot ID: Q14515
• Protein Accession #: NP_001121782.1
• Nucleotide Accession #: NM_001128310.2