SOD3 ELISA Kit (Rat) (OKEH03478)

Catalog No: OKEH03478

Size:96T

Rat SOD3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SOD3 ELISA Kit (Rat) (OKEH03478) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sod3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Sod3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sod3 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: <= 5.2%
• ELISA Kit Component:
  • Anti-Sod3 Microplate: 96 Wells (12 x 8 Well strips)
  • Sod3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Sod3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 7.12 pg/mL
• Specificity: Natural and recombinant Rat Extracellular superoxide dismutase [Cu-Zn]
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sod3
• Gene Full Name: superoxide dismutase 3
• Alias Symbols: EC-SOD;ECSODPT;extracellular superoxide dismutase;extracellular superoxide dismutase [Cu-Zn];Superoxide dimutase 3;superoxide dismutase 3, extracellular;superoxide dismutase B.
• NCBI Gene Id: 25352
• Protein Name: Q08420
• Description of Target: Extracellular superoxide dismutase [Cu-Zn]
• Uniprot ID: https://www.uniprot.org/uniprot/Q08420
• Protein Accession #: NP_037012.1
• Nucleotide Accession #: NM_012880.1
• Protein Size (# AA): 244
• Molecular Weight: 27 kDa