SOCS1 Antibody (OAEB02451)

Catalog No: OAEB02451 (Formerly GWB-42CF61)

Size:100UG

HEK293 overexpressing SOCS1 and probed with OAEB02451 (mock transfection in lane B).

OAEB02451 staining (2ug/ml) of mouse spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02451 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SOCS1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-VLRDYLSSFPFQI
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:4000.
  Western Blot: In transfected HEK293 transiently expressing human SOCS1 bands of approx. 30kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. A similar pattern is observed in Mouse Spleen lysates. The calculated molecular size is 23.6kDa according to Human NP_003736.1 and 23.7kDa according to Mouse NP_034026.1. Recommended concentration, 1-3ug/ml.
  Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of the Brush Border in PCT. Recommended concentration, 2-3ug/ml.
• Reference: * Starr R, Willson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ.; A family of cytokine-inducible inhibitors of signalling.; Nature 1997 Jun 26;387(6636):917-21; Pubmed 9202125

Target Info

• Gene Symbol: Socs1|SOCS1
• Gene Full Name: suppressor of cytokine signaling 1
• Alias Symbols: AISIMD;Cis;CIS1;Cish1;Cish7;cytokine inducible SH2-containing protein 1;cytokine inducible SH2-containing protein 7;cytokine-inducible SH2 protein 1;JA;JAB;JAK binding protein;JAK2-binding protein;SOC;SOCS-1;SS;SSI1;SSI-1;STAT induced SH3 protein 1;STAT-induced STAT inhibitor 1;suppressor of cytokine signaling 1;Tec-interacting protein 3;TIP3;TIP-3.
• NCBI Gene Id: 12703|8651
• Protein Name: Suppressor of cytokine signaling 1
• Uniprot ID: O15524
• Protein Accession #: NP_003736.1
• Molecular Weight: 23.6