Catalog No: OKEH03912
Size:96T
Price: $625.00
SKU
OKEH03912
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SNCA ELISA Kit (Pig) (OKEH03912) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNCA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNCA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNCA captured in well.
ELISA Kit Component
ComponentAmount
SNCA Microplate96 Wells (12 x 8 Well strips)
SNCA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SNCA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
SpecificityNatural and recombinant Pig Alpha-synuclein
Assay InfoAssay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.
Gene SymbolSNCA
Gene Full Namesynuclein alpha
Alias Symbolsalpha-synuclein.
NCBI Gene Id641350
Protein NameQ3I5G7
Description of TargetAlpha-synuclein
Uniprot IDhttps://www.uniprot.org/uniprot/Q3I5G7
Protein Accession #NP_001032222.1
Nucleotide Accession #NM_001037145.1
Protein Size (# AA)140
Molecular Weight140 kDa
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