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Datasheets/Manuals | Printable datasheet for SMURF2 Antibody - N-terminal (OAPB01891) (OAPB01891) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2. The immunogen is located within amino acids 140 - 190 of SMURF2. |
Purification | Affinity Purified |
Concentration | 1 mg/ml |
Gene Symbol | SMURF2 |
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Gene Full Name | SMAD specific E3 ubiquitin protein ligase 2 |
Alias Symbols | E3 ubiquitin ligase SMURF2;E3 ubiquitin-protein ligase SMURF2;HECT-type E3 ubiquitin transferase SMURF2;hSMURF2;SMAD ubiquitination regulatory factor 2;SMAD-specific E3 ubiquitin-protein ligase 2. |
NCBI Gene Id | 64750 |
Protein Name | E3 ubiquitin-protein ligase SMURF2|E3 ubiquitin-protein ligase SMURF2 [Homo sapiens] |
Description of Target | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
Uniprot ID | Q9HAU4 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_073576 |
Nucleotide Accession # | NM_022739.3 |
Molecular Weight | Predicted: 82 kDa Observed: 85 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SMURF2 Antibody - N-terminal (OAPB01891)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "SMURF2 Antibody - N-terminal (OAPB01891)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "SMURF2 Antibody - N-terminal (OAPB01891)" provided in?
This item is provided in "Liquid. SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SMURF2 Antibody - N-terminal (OAPB01891)"?
This target may also be called "E3 ubiquitin ligase SMURF2;E3 ubiquitin-protein ligase SMURF2;HECT-type E3 ubiquitin transferase SMURF2;hSMURF2;SMAD ubiquitination regulatory factor 2;SMAD-specific E3 ubiquitin-protein ligase 2." in publications.
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What is the shipping cost for "SMURF2 Antibody - N-terminal (OAPB01891)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SMURF2 Antibody - N-terminal (OAPB01891)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SMURF2 Antibody - N-terminal (OAPB01891)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 82 kDa Observed: 85 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SMURF2 Antibody - N-terminal (OAPB01891)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SMURF2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SMURF2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SMURF2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SMURF2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SMURF2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SMURF2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.