SMURF2 Antibody - C-terminal region (OAAB15694)

Catalog No: OAAB15694

Size:400ul

Western blot analysis of anti-SMURF2 Pab (Cat. # OAAB15694) in 293 cell line lysate (35ug/lane). SMURF2(arrow) was detected using the purified Pab (1:30 dilution)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30;282(48) :35259-35268)

Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). Transfected cells were analyzed at 3 d.i.v. by staining with an anti-Smurf2 antibody (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 ?. (J. Biol. Chem. 2007 Nov 30;282(48) :35259-35268)

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: SMURF2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level
  Cellular Location: Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts
  Tissue Location: Widely expressed.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 amino acids from the C-terminal region of human SMURF2.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000
  IHC-P: 1:50~100
  IF: 1:10~50

Target Info

• Gene Symbol: SMURF2
• Alias Symbols: SMURF2, E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2
• NCBI Gene Id: 64750
• Protein Name: E3 ubiquitin-protein ligase SMURF2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9HAU4
• Protein Accession #: NP_073576.1
• Molecular Weight: 86196 Da