Catalog No: OKEH03346
Size:96T
Price: $525.00
SKU
OKEH03346
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SMPD1 ELISA Kit (Mouse) (OKEH03346) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Smpd1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Smpd1 and incubated. The Smpd1 found in the sample or standards competes with the biotinylated Smpd1 for limited binding sites on the immobilized anti-Mouse Smpd1 antibody. Excess unbound biotinylated Smpd1 and sample or standard Smpd1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Smpd1 captured in the well and inversely proportional to the amount of Smpd1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/ml | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 6.9% (n = 20) Mean Inter-assay CV%: <= 8.8% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.156 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse Sphingomyelin phosphodiesterase | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Smpd1 |
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Gene Full Name | sphingomyelin phosphodiesterase 1, acid lysosomal |
Alias Symbols | A;acid sphingomyelinase;aS;A-S;ASM;aSMase;A-SMase;sphingomyelin phosphodiesterase;Zn-S;Zn-SMase. |
NCBI Gene Id | 20597 |
Protein Name | Q04519 |
Description of Target | Sphingomyelin phosphodiesterase |
Uniprot ID | https://www.uniprot.org/uniprot/Q04519 |
Protein Accession # | NP_035551.1 |
Nucleotide Accession # | NM_011421.2 |
Protein Size (# AA) | 627 |
Molecular Weight | 70 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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