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Catalog No: OKCD00172 (Formerly GWB-KBBTU7)
Size:96WELLS
Price: $650.00
SKU
OKCD00172
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Datasheets/ManualsPrintable datasheet for SLPI ELISA Kit (Mouse) (OKCD00172)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)81-99%78-101%80-97%97-105%
EDTA plasma(n=5)93-101%91-98%78-95%92-101%
heparin plasma(n=5)80-88%85-101%92-101%87-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretory Leukocyte Peptidase Inhibitor (SLPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secretory Leukocyte Peptidase Inhibitor (SLPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-8983
EDTA plasma(n=5)94-10197
heparin plasma(n=5)91-9995
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Slpi Microplate96 Wells (12 x 8 Well Strips)
Slpi Lyophilized Standard2 x 1 ng
100X Biotinylated Slpi Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, urine, cell culture supernates and other biological fluids
Sensitivity< 6.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretory Leukocyte Peptidase Inhibitor (SLPI). No significant cross-reactivity or interference between Secretory Leukocyte Peptidase Inhibitor (SLPI) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSlpi
Gene Full Namesecretory leukocyte peptidase inhibitor
Alias SymbolsALP;antileukoproteinase;secretory leukocyte protease inhibitor.
NCBI Gene Id20568
Protein NameAntileukoproteinase
Description of TargetAcid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:9126337). Modulates the innate immune response after bacterial infection (PubMed:12615907). Contributes to regulate the inflammatory and immune responses to the intracellular parasite L.major (PubMed:25030421). Down-regulates responses to bacterial lipopolysaccharide (LPS) (PubMed:9039268, PubMed:12615907, PubMed:25030421). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:11017147, PubMed:12615907). Has antimicrobial activity against mycobacteria, but not against salmonella (PubMed:18322212). Contributes to normal resistance against infection by M.tuberculosis (PubMed:18322212). Required for normal resistance to L.major (PubMed:25030421). Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (PubMed:11017147, PubMed:25030421). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (By similarity).
Uniprot IDP97430
Protein Accession #NP_035544
Nucleotide Accession #NM_011414.3
Protein Size (# AA)131
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