Slit3 ELISA Kit (Human) (OKDD00532)

Catalog No: OKDD00532 (Formerly GWB-KBBIF7)

Size:96 Wells

Human Slit3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Slit3 ELISA Kit (Human) (OKDD00532) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for Slit3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slit3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Slit3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slit3 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slit3 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-Slit3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Slit3 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X Slit3 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Slit3. No significant cross-reactivity or interference between Slit3 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLIT3
• Gene Full Name: slit guidance ligand 3
• Alias Symbols: MEGF5;multiple EGF-like domains protein 5;multiple epidermal growth factor-like domains protein 5;SLIL2;slit homolog 3;slit homolog 3 protein;SLIT1;slit2;Slit-3.
• NCBI Gene Id: 6586
• Protein Name: Slit homolog 3 protein
• Description of Target: May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
• Uniprot ID: O75094
• Protein Accession #: NP_001258875.1
• Nucleotide Accession #: NM_001271946.1
• Protein Size (# AA): 1523
• Molecular Weight: 168 kDa