Slit1 ELISA Kit (Mouse) (OKDD00725)

Catalog No: OKDD00725 (Formerly GWB-KBBDB7)

Size:96 Wells

Mouse Slit1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Slit1 ELISA Kit (Mouse) (OKDD00725) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Slit1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slit1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Slit1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-Slit1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Slit1 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X Slit1 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates
• Sensitivity: <0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Slit1. No significant cross-reactivity or interference between Slit1 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Slit1
• Gene Full Name: slit guidance ligand 1
• Alias Symbols: mKIAA0813;S;Slil1;slit homolog 1 protein;slit-1.
• NCBI Gene Id: 20562
• Protein Name: Slit homolog 1 protein
• Description of Target: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
• Uniprot ID: Q80TR4
• Protein Accession #: NP_035542.2
• Nucleotide Accession #: NM_011412.3
• Protein Size (# AA): 1523
• Molecular Weight: 168 kDa