SLIT1 ELISA Kit (Human) (OKCD00383)

Catalog No: OKCD00383 (Formerly GWB-KBBLB6)

Size:96WELLS

Human SLIT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	88-95%	81-97%	94-101%	86-94%
  EDTA plasma(n=5)	79-97%	88-101%	86-94%	90-102%
  heparin plasma(n=5)	97-105%	85-99%	91-99%	91-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slit Homolog 1 (Slit1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Slit Homolog 1 (Slit1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slit Homolog 1 (Slit1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Slit Homolog 1 (Slit1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	89-103	93
  EDTA plasma(n=5)	84-92	87
  heparin plasma(n=5)	85-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slit Homolog 1 (Slit1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slit Homolog 1 (Slit1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLIT1 Microplate: 96 Wells (12 x 8 Well Strips)
  • SLIT1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SLIT1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Slit Homolog 1 (Slit1). No significant cross-reactivity or interference between Slit Homolog 1 (Slit1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SLIT1
• Gene Full Name: slit guidance ligand 1
• Alias Symbols: MEGF4;multiple EGF-like domains protein 4;multiple epidermal growth factor-like domains protein 4;SLIL1;slit homolog 1;slit homolog 1 protein;SLIT-1;SLIT3.
• NCBI Gene Id: 6585
• Protein Name: Slit homolog 1 protein
• Description of Target: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
• Uniprot ID: O75093
• Protein Accession #: NP_003052
• Nucleotide Accession #: NM_003061.2
• Protein Size (# AA): 1534