Catalog No: OKEH02450
Size:96T
Price: $625.00
SKU
OKEH02450
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC50A1 ELISA Kit (Human) (OKEH02450) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC50A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC50A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC50A1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
SLC50A1 Microplate96 Wells (12 x 8 Well strips)
SLC50A1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SLC50A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.098 ng/mL
SpecificityNatural and recombinant Human Sugar transporter SWEET1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSLC50A1
Gene Full Namesolute carrier family 50 member 1
Alias SymbolsHsSWEET1;probable sugar transporter RAG1AP1;RAG1-activating protein 1;RAG1AP1;recombination activating gene 1 activating protein 1;RP11-540D14.5;RZPDo834D038D;SCP;slv;solute carrier family 50 (sugar efflux transporter), member 1;solute carrier family 50 (sugar transporter), member 1;Solute carrier family 50 member 1;stromal cell protein;sugar transporter SWEET1;SWEET1.
NCBI Gene Id55974
Protein NameQ9BRV3
Description of TargetSugar transporter SWEET1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BRV3
Protein Accession #NP_001116309.1
Nucleotide Accession #NM_001122837.1
Protein Size (# AA)221
Molecular Weight25 kDa
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