Catalog No: OKEH03087
Size:96T
Price: $525.00
SKU
OKEH03087
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC30A8 ELISA Kit (Rat) (OKEH03087) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc30a8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Slc30a8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc30a8 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Component
ComponentAmount
Anti-Slc30a8 Microplate96 Wells (12 x 8 Well strips)
Slc30a8 Lyophilized Standard2
100X Biotinylated Slc30a8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.423 ng/mL
SpecificityNatural and recombinant Rat Zinc transporter 8
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSlc30a8
Gene Full Namesolute carrier family 30 member 8
Alias Symbolssolute carrier family 30 (zinc transporter), member 8;Solute carrier family 30 member 8;zinc transporter 8;ZnT-8.
NCBI Gene Id299903
Protein NameP0CE46
Description of TargetZinc transporter 8
Uniprot IDhttps://www.uniprot.org/uniprot/P0CE46
Protein Accession #NP_001124010.1
Nucleotide Accession #NM_001130538.1
Protein Size (# AA)368
Molecular Weight40 kDa
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