SLC30A3 ELISA Kit (Human) (OKEH02261)

Catalog No: OKEH02261

Size:96T

Human SLC30A3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SLC30A3 ELISA Kit (Human) (OKEH02261) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human SLC30A3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SLC30A3 and incubated. The SLC30A3 found in the sample or standards competes with the biotinylated SLC30A3 for limited binding sites on the immobilized anti-Human SLC30A3 antibody. Excess unbound biotinylated SLC30A3 and sample or standard SLC30A3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SLC30A3 captured in the well and inversely proportional to the amount of SLC30A3 which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.6% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-SLC30A3 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC30A3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X SLC30A3 Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.098 ng/mL
• Specificity: Natural and recombinant Human Zinc transporter 3
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: SLC30A3
• Gene Full Name: solute carrier family 30 member 3
• Alias Symbols: solute carrier family 30 (zinc transporter), member 3;Solute carrier family 30 member 3;zinc transporter 3;zinc transporter ZnT-3;znT-3;ZNT3.
• NCBI Gene Id: 7781
• Protein Name: Q99726
• Description of Target: Zinc transporter 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q99726
• Protein Accession #: NP_001305878.1
• Nucleotide Accession #: NM_001318949.1
• Protein Size (# AA): 388
• Molecular Weight: 42 kDa