SLC25A4 ELISA Kit (Rat) (OKEH04195)

Catalog No: OKEH04195

Size:96T

Rat SLC25A4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Slc25a4 ELISA Kit (Rat) (OKEH04195) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc25a4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc25a4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc25a4 captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Slc25a4 Microplate: 96 Wells (12 x 8 Well Strips)
  • Slc25a4 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Slc25a4 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.092 ng/mL
• Specificity: Natural and recombinant Rat ADP/ATP translocase 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Slc25a4
• Gene Full Name: solute carrier family 25 member 4
• Alias Symbols: adenine nucleotide translocator 1;adenine nucleotide translocator), member 4;ADP,ATP carrier protein 1;ADP/ATP translocase 1;ANT 1;Ant1;mitochondrial adenine nucleotide translocator;solute carrier family 25 (mitochondrial adenine nucleotide translocator) member 4;solute carrier family 25 (mitochondrial carrier;solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4;Solute carrier family 25 member 4.
• NCBI Gene Id: 85333
• Protein Name: Q05962
• Description of Target: ADP/ATP translocase 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q05962
• Protein Accession #: NP_445967.1
• Nucleotide Accession #: NM_053515.1
• Protein Size (# AA): 298
• Molecular Weight: 33 kDa