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Catalog No: OKCD01211 (Formerly GWB-KBBIZ5)
Size:96WELLS
Price: $675.00
SKU
OKCD01211
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Datasheets/ManualsPrintable datasheet for SLC22A4 ELISA Kit (Human) (OKCD01211)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
91-104%97-105%97-104%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Organic Cation/Ergothioneine Transporter (OCTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Organic Cation/Ergothioneine Transporter (OCTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Organic Cation/Ergothioneine Transporter (OCTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Organic Cation/Ergothioneine Transporter (OCTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
97-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Organic Cation/Ergothioneine Transporter (OCTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Organic Cation/Ergothioneine Transporter (OCTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SLC22A4 Microplate96 Wells (12 x 8 Well Strips)
SLC22A4 Lyophilized Standard2 x 5 ng
100X Biotinylated SLC22A4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 27 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Organic Cation/Ergothioneine Transporter (OCTN1). No significant cross-reactivity or interference between Organic Cation/Ergothioneine Transporter (OCTN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSLC22A4
Gene Full Namesolute carrier family 22 member 4
Alias Symbolsdeafness, autosomal recessive 60;DFNB60;ergothioneine transporter;ET transporter;integral membrane transport protein;OCTN1;organic cation/carnitine transporter 1;solute carrier family 22 (organic cation/ergothioneine transporter), member 4;solute carrier family 22 (organic cation/zwitterion transporter), member 4;solute carrier family 22 member 4.
NCBI Gene Id6583
Protein NameSolute carrier family 22 member 4
Description of TargetSodium-ion dependent, low affinity carnitine transporter. Probably transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 1.78. A key substrate of this transporter seems to be ergothioneine (ET).
Uniprot IDQ9H015
Protein Accession #NP_003050
Nucleotide Accession #NM_003059.2
Protein Size (# AA)551
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