SLC1A5 ELISA Kit (Human) (OKCD00935)

Catalog No: OKCD00935 (Formerly GWB-KBBMG3)

Size:96WELLS

Human SLC1A5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 1, Member 5 (SLC1A5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 1, Member 5 (SLC1A5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 1, Member 5 (SLC1A5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Solute Carrier Family 1, Member 5 (SLC1A5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 1, Member 5 (SLC1A5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 1, Member 5 (SLC1A5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLC1A5 Microplate: 96 Wells (12 x 8 Well Strips)
  • SLC1A5 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated SLC1A5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 1, Member 5 (SLC1A5). No significant cross-reactivity or interference between Solute Carrier Family 1, Member 5 (SLC1A5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SLC1A5
• Gene Full Name: solute carrier family 1 member 5
• Alias Symbols: AAAT;ASCT2;ATB(0);ATBO;baboon M7 virus receptor;M7V1;M7VS1;neutral amino acid transporter B;neutral amino acid transporter B(0);R16;RD114 virus receptor;RD114/simian type D retrovirus receptor;RDRC;sodium-dependent neutral amino acid transporter type 2;solute carrier family 1 (neutral amino acid transporter), member 5;Solute carrier family 1 member 5.
• NCBI Gene Id: 6510
• Protein Name: Neutral amino acid transporter B(0)
• Description of Target: Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids (PubMed:8702519). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904).
• Uniprot ID: Q15758
• Protein Accession #: NP_001138616
• Nucleotide Accession #: NM_001145144.1
• Protein Size (# AA): 521