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Catalog No: OKCD00286 (Formerly GWB-KBBLX8)
Size:96WELLS
Price: $650.00
SKU
OKCD00286
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Datasheets/ManualsPrintable datasheet for SLC12A7 ELISA Kit (Human) (OKCD00286)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Chloride Cotransporters 4 (KCC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Chloride Cotransporters 4 (KCC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Chloride Cotransporters 4 (KCC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Potassium Chloride Cotransporters 4 (KCC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Potassium Chloride Cotransporters 4 (KCC4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Potassium Chloride Cotransporters 4 (KCC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SLC12A7 Microplate96 Wells (12 x 8 Well Strips)
SLC12A7 Lyophilized Standard2 x 10 ng
100X Biotinylated SLC12A7 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Potassium Chloride Cotransporters 4 (KCC4). No significant cross-reactivity or interference between Potassium Chloride Cotransporters 4 (KCC4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSLC12A7
Gene Full Namesolute carrier family 12 member 7
Alias Symbolselectroneutral potassium-chloride cotransporter 4;KCC4;K-Cl cotransporter 4;potassium/chloride transporter KCC4;solute carrier family 12 (potassium/chloride transporter), member 7;solute carrier family 12 (potassium/chloride transporters), member 7;solute carrier family 12 member 7;SUCO/SLC12A7 fusion.
NCBI Gene Id10723
Protein NameSolute carrier family 12 member 7
Description of TargetMediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).
Uniprot IDQ9Y666
Protein Accession #NP_006589
Nucleotide Accession #NM_006598.2
Protein Size (# AA)1083
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