SLC11A2 ELISA Kit (Rat) (OKEH04285)

Catalog No: OKEH04285

Size:96T

Rat SLC11A2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Slc11a2 ELISA Kit (Rat) (OKEH04285) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc11a2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc11a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc11a2 captured in the well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Component:
  • Slc11a2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Slc11a2 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Slc11a2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 7.9 pg/mL
• Specificity: Natural and recombinant Rat Natural resistance-associated macrophage protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Slc11a2
• Gene Full Name: solute carrier family 11 member 2
• Alias Symbols: divalent cation transporter 1;divalent metal transporter 1;Dmt1;DMT-1;natural resistance-associated macrophage protein 2;NRAMP 2;Nramp2;solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2;solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2;Solute carrier family 11 member 2;Solute carrier family 11 member 2 (natural resistance-associated macrophage protein 2).
• NCBI Gene Id: 25715
• Protein Name: O54902
• Description of Target: Natural resistance-associated macrophage protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/O54902
• Protein Accession #: NP_037305.2
• Nucleotide Accession #: NM_013173.2
• Protein Size (# AA): 568
• Molecular Weight: 62 kDa