SIRT6 ELISA Kit (Mouse) (OKCD02892)

Catalog No: OKCD02892

Size:96WELLS

Mouse SIRT6 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 6 (SIRT6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sirtuin 6 (SIRT6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 6 (SIRT6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sirtuin 6 (SIRT6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 6 (SIRT6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 6 (SIRT6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SIRT6 Microplate: 96 Wells (12 x 8 Well strips)
  • SIRT6 Lyophilized Standard: 2
  • 100X Biotinylated SIRT6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sirtuin 6 (SIRT6). No significant cross-reactivity or interference between Sirtuin 6 (SIRT6) and analogues was observed.
• Publications:

  Dichotomous responses to chronic fetal hypoxia lead to a predetermined aging phenotype. Mol Cell Proteomics. 100190 (2021). 34958949

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for SIRT6 ELISA kit (Mouse) on CiteAb

Target Info

• Gene Symbol: Sirt6
• Gene Full Name: sirtuin 6
• Alias Symbols: 2810449N18Rik;AI043036;NAD-dependent deacetylase sirtuin-6;NAD-dependent protein deacetylase sirtuin-6;regulatory protein SIR2 homolog 6;Sir2l6;SIR2-like protein 6.
• NCBI Gene Id: 50721
• Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Description of Target: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate (By similarity). Acts as a corepressor of the transcription factor Hif1a to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. Regulates the production of TNF protein. Has a role in the regulation of life span in male mice, but not in female mice.
• Uniprot ID: P59941
• Protein Accession #: NP_853617.1
• Nucleotide Accession #: NM_181586.3
• Protein Size (# AA): 334