SIRT3 Antibody (OACA07062)

Catalog No: OACA07062

Immunohistochemistry of paraffin-embedded breast cancer prostate at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Immunohistochemistry of paraffin-embedded human pancreas at dilution 1:100

All lanes: NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody at 2ug/ml
Lane 1: NIH3T3 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 44,29 kDa
Observed band size: 44 kDa

All lanes: NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody at 2ug/ml
Lane 1: NIH3T3 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 44,29 kDa
Observed band size: 44 kDa

Immunohistochemistry of paraffin-embedded breast cancer prostate at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Immunohistochemistry of paraffin-embedded human pancreas at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial protein (20-130AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"Structures of human sirtuin 3 complexes with ADP-ribose and with carba-NAD+ and SRT1720: binding details and inhibition mechanism."Nguyen G.T., Schaefer S., Gertz M., Weyand M., Steegborn C.Acta Crystallogr. D 69:1423-1432(2013). [2]"Crystal structures of human SIRT3 displaying substrate-induced conformational changes."Jin L., Wei W., Jiang Y., Peng H., Cai J., Mao C., Dai H., Choy W., Bemis J.E., Jirousek M.R., Milne J.C., Westphal C.H., Perni R.B.J. Biol. Chem. 284:24394-24405(2009). [3]"SIRT3 regulates long-chain acyl-coA dehydrogenase by deacetylating conserved lysines near the active site."Bharathi S.S., Zhang Y., Mohsen A.W., Uppala R., Balasubramani M., Schreiber E., Uechi G., Beck M.E., Rardin M.J., Vockley J., Verdin E., Gibson B.W., Hirschey M.D., Goetzman E.S.J. Biol. Chem. 288:33837-33847(2013).

Target Info

• Gene Symbol: SIRT3
• Gene Full Name: sirtuin 3
• Alias Symbols: mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase;NAD-dependent deacetylase sirtuin-3, mitochondrial;NAD-dependent protein deacetylase sirtuin-3, mitochondrial;regulatory protein SIR2 homolog 3;silent mating type information regulation 2, S.cerevisiae, homolog 3;SIR2L3;sir2-like 3;SIR2-like protein 3;sirtuin type 3.
• NCBI Gene Id: 23410
• Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Description of Target: NAD-dependent protein deacetylase (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090, PubMed:19535340). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O (PubMed:16788062, PubMed:18680753, PubMed:24121500, PubMed:24252090, PubMed:19535340). Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels (PubMed:18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).
• Uniprot ID: Q9NTG7
• Protein Accession #: NP_001017524
• Nucleotide Accession #: NM_001017524