SIRT3 Antibody - middle region (OAEB02815)

Catalog No: OAEB02815 (Formerly GWB-EV1203)

Size:100UG

OAEB02815 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: Sirt3
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: C-RASGIPASKLVEAH
• Concentration: 0.5 mg/ml
• Epitope: middle region
• Application Info: Western Blot: Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide.
  Peptide ELISA: antibody detection limit dilution 1:64000.
  

Target Info

• Gene Symbol: Sirt3|SIRT3
• Gene Full Name: sirtuin 3
• Alias Symbols: 2310003L23Rik;AI848213;mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase;mitochondrial protein lysine deacetylase;mSIR2L3;NAD-dependent deacetylase sirtuin-3;NAD-dependent deacetylase sirtuin-3, mitochondrial;NAD-dependent protein deacetylase sirtuin-3;NAD-dependent protein deacetylase sirtuin-3, mitochondrial;regulatory protein SIR2 homolog 3;Si;silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3;silent mating type information regulation 2, S.cerevisiae, homolog 3;Sir2l3;sir2-like 3;SIR2-like protein 3;SIRT3L mitochondrial;sirtuin type 3.
• NCBI Gene Id: 23410|293615|64384
• Protein Accession #: NP_071878.2;
  NP_001171275.1
• Molecular Weight: 28.8; 36.6