SIRT2 ELISA Kit (Rat) (OKEH03114)

Catalog No: OKEH03114

Size:96T

Rat SIRT2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SIRT2 ELISA Kit (Rat) (OKEH03114) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sirt2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Sirt2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sirt2 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • Anti-Sirt2 Microplate: 96 Wells (12 x 8 Well strips)
  • Sirt2 Lyophilized Standard: 2
  • 100X Biotinylated Sirt2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.157 ng/mL
• Specificity: Natural and recombinant Rat NAD-dependent deacetylase sirtuin-2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sirt2
• Gene Full Name: sirtuin 2
• Alias Symbols: 5E5 antigen;NAD-dependent deacetylase sirtuin-2;NAD-dependent protein deacetylase sirtuin-2;regulatory protein SIR2 homolog 2;SIR2-like protein 2.
• NCBI Gene Id: 361532
• Protein Name: Q5RJQ4
• Description of Target: NAD-dependent protein deacetylase sirtuin-2
• Uniprot ID: https://www.uniprot.org/uniprot/Q5RJQ4
• Protein Accession #: NP_001008369.1
• Nucleotide Accession #: NM_001008368.1
• Protein Size (# AA): 350
• Molecular Weight: 39 kDa