Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01706
Size:96T
Price: $525.00
SKU
OKEH01706
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SIK2 ELISA Kit (Human) (OKEH01706) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SIK2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SIK2 and incubated. The SIK2 found in the sample or standards competes with the biotinylated SIK2 for limited binding sites on the immobilized anti-human SIK2 antibody. Excess unbound biotinylated SIK2 and sample or standard SIK2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SIK2 captured in the well and inversely proportional to the amount of SIK2 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-SIK2 Microplate96 Wells (12 x 8 Well strips)
SIK2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X SIK2-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.15 ng/mL
SpecificityNatural and recombinant Human Serine/threonine-protein kinase SIK2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolSIK2
Gene Full Namesalt inducible kinase 2
Alias SymbolsLOH11CR1I;QIK;qin-induced kinase;Salt-inducible kinase 2;salt-inducible protein kinase 2;salt-inducible serine/threonine kinase 2;serine/threonine-protein kinase SIK2;serine/threonine-protein kinase SNF1-like kinase 2;SIK-2;SNF1-like kinase 2;SNF1LK2.
NCBI Gene Id23235
Protein NameQ9H0K1
Description of TargetSerine/threonine-protein kinase SIK2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9H0K1
Protein Accession #NP_056006.1
Nucleotide Accession #NM_015191.2
Protein Size (# AA)926
Molecular Weight103 kDa
Write Your Own Review
You're reviewing:SIK2 ELISA Kit (Human) (OKEH01706)
Your Rating
We found other products you might like!