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Now Offering Over 85,203 Antibodies & 34,301 Antigens!

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100 ul
$289.00
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SH3RF1 antibody - middle region (ARP43283_P050)

Description of Target:
SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.
Gene Symbol:
SH3RF1
Official Gene Full Name:
SH3 domain containing ring finger 1
NCBI Gene Id:
57630
Alias Symbols:
FLJ21602; KIAA1494; POSH; RNF142; SH3MD2
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SH3RF1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SH3RF1.
Swissprot Id:
Q7Z6J0
Protein Accession #:
NP_065921
Nucleotide Accession #:
NM_020870
Host:
Rabbit
Clonality:
Polyclonal
Protein Name:
E3 ubiquitin-protein ligase SH3RF1
Protein Size (# AA):
888
Molecular Weight:
93kDa
Application:
WB
Protein Interactions:
YWHAB; UBE2N; UBE2D3; UBE2D1; AKT2; AKT1; SIAH2; SIAH1; SH3RF2; RAC1; SEMA6A; UBC; PDCD6IP; KCNJ1; MAP4K3; MAP3K9; ADAM15; PAK2; RACGAP1; MAP3K12; MAP3K10; MAP3K11;
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human SH3RF1
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Purification:
Affinity Purified
Complete computational species homology data:
Anti-SH3RF1 (ARP43283_P050)
Predicted Homology Based on Immunogen Sequence:
Pig: 100%; Rat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Guinea pig: 100%; Dog: 93%; Bovine: 93%
Species Reactivity:
Mouse, Horse, Rabbit, Guinea pig, Rat, Human, Bovine, Dog
Datasheets / Downloads:
Printable datasheet for anti-SH3RF1 (ARP43283_P050) antibody
Peptide Sequence:
Synthetic peptide located within the following region: LLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHG
Blocking Peptide:
For anti-SH3RF1 (ARP43283_P050) antibody is Catalog # AAP43283 (Previous Catalog # AAPP11358)
Target Reference:
Lyons,T.R., (2007) J. Biol. Chem. 282 (30), 21987-21997
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Product Protocols: SH3RF1 antibody tested with Human Du145 Cells (ARP43283_P050)

Aviva Systems Biology is the original manufacturer of this SH3RF1 antibody (ARP43283_P050)

Click here to view the SH3RF1 antibody Western Blot Protocol

Product Datasheet Link: SH3RF1 antibody (ARP43283_P050)

WB Suggested Anti-SH3RF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: DU145

Western Blot image:


Description of Target: SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s SH3RF1 antibody (ARP43283_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
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