Catalog No: OKDD00523 (Formerly GWB-KBBMD4)
Size:96 Wells
Price: $800.00
SKU
OKDD00523
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for SGSH ELISA Kit (Human) (OKDD00523) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SGSH ELISA Kit (Human) (OKDD00523) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for SGSH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SGSH is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SGSH captured in well. | ||||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SGSH were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SGSH were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10%
Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, cell lysates | ||||||||||||||||||||||||
Sensitivity | < 0.054 ng/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of SGSH. No significant cross-reactivity or interference between SGSH and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | SGSH |
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Gene Full Name | N-sulfoglucosamine sulfohydrolase |
Alias Symbols | heparan sulfate sulfatase;HSS;MPS3A;mucopolysaccharidosis type IIIA;N-sulphoglucosamine sulphohydrolase;SFMD;sulfoglucosamine sulfamidase;sulphamidase. |
NCBI Gene Id | 6448 |
Protein Name | N-sulphoglucosamine sulphohydrolase |
Description of Target | Catalyzes a step in lysosomal heparan sulfate degradation. |
Uniprot ID | P51688 |
Protein Accession # | NP_000190.1 |
Nucleotide Accession # | NM_000199.3 |
Protein Size (# AA) | 502 |
Molecular Weight | 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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