Catalog No: OKEH01170
Size:96T
Price: $600.00
SKU
OKEH01170
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SFTPC ELISA Kit (Mouse) (OKEH01170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFTPC captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 10.4% (n = 20)
ELISA Kit Component
ComponentAmount
SFTPC Microplate96 Wells (12 x 8 Well strips)
SFTPC Lyophilized Standard2
100X Biotinylated SFTPC Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity29.5 pg/mL
SpecificityNatural and recombinant Mouse Pulmonary surfactant-associated protein C
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolSFTPC
Gene Full Namesurfactant associated protein C
Alias SymbolsPulmonary surfactant-associated proteolipid SPL(Val);SP5.
NCBI Gene Id20389
Protein NameP21841
Description of TargetPulmonary surfactant-associated protein C
Uniprot IDhttps://www.uniprot.org/uniprot/P21841
Protein Accession #NP_035489.2
Nucleotide Accession #NM_011359.2
Protein Size (# AA)193
Molecular Weight21 kDa
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