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Catalog No: OKCD01375
Size:96WELLS
Price: $750.00
SKU
OKCD01375
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SFRP5 High Sensitivity ELISA Kit (Human) (OKCD01375)

Datasheets/ManualsPrintable datasheet for SFRP5 High Sensitivity ELISA Kit (Human) (OKCD01375)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)83-104%78-102%85-94%82-93%
EDTA Plasma (n=5)87-101%82-102%81-96%79-95%
Heparin Plasma (n=5)93-101%84-97%78-101%89-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Secreted Frizzled Related Protein 5 (SFRP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Secreted Frizzled Related Protein 5 (SFRP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Secreted Frizzled Related Protein 5 (SFRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Secreted Frizzled Related Protein 5 (SFRP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10494
EDTA Plasma (n=5)96-105101
Heparin Plasma (n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Secreted Frizzled Related Protein 5 (SFRP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Secreted Frizzled Related Protein 5 (SFRP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SFRP5 Microplate96 Wells (12 x 8 Well Strips)
SFRP5 Lyophilized Standard2
100X Biotinylated SFRP5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity0.51 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 5 (SFRP5). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSFRP5
Gene Full Namesecreted frizzled related protein 5
Alias Symbolsfrizzled-related protein 1b;FRP-1b;SARP3;SARP-3;secreted apoptosis related protein 3;Secreted apoptosis-related protein 3;secreted frizzled-related protein 5;sFRP-5.
NCBI Gene Id6425
Protein NameSecreted frizzled-related protein 5
Description of TargetSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.
Uniprot IDQ5T4F7
Protein Accession #NP_003006.2
Nucleotide Accession #NM_003015.3
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