SF3B3 ELISA Kit (Mouse) (OKCD00616)

Catalog No: OKCD00616 (Formerly GWB-KBBKW7)

Size:96WELLS

Mouse Sf3b3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Splicing Factor 3B Subunit 3 (SF3B3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Splicing Factor 3B Subunit 3 (SF3B3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Sf3b3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Sf3b3 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Sf3b3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Splicing Factor 3B Subunit 3 (SF3B3). No significant cross-reactivity or interference between Splicing Factor 3B Subunit 3 (SF3B3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Sf3b3
• Gene Full Name: splicing factor 3b, subunit 3
• Alias Symbols: 1810061H24Rik;5730409A01Rik;AA409318;D8Ertd633;D8Ertd633e;mKIAA0017;pre-mRNA-splicing factor SF3b 130 kDa subunit;RS;RSE1;SAP;SAP 130;SAP130;SF3b130;spliceosome-associated protein 130;splicing factor 3B subunit 3.
• NCBI Gene Id: 101943
• Protein Name: Splicing factor 3B subunit 3
• Description of Target: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
• Uniprot ID: Q921M3
• Protein Accession #: NP_598714
• Nucleotide Accession #: NM_133953.2
• Protein Size (# AA): 1217