SERPINI1 ELISA Kit (Human) (OKCD01254)

Catalog No: OKCD01254 (Formerly GWB-KBBOA5)

Size:96WELLS

Human SERPINI1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-103%	94-105%	81-96%	78-98%
  EDTA plasma(n=5)	79-102%	88-102%	89-102%	85-93%
  heparin plasma(n=5)	95-102%	97-105%	97-104%	86-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroserpin (NSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroserpin (NSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroserpin (NSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuroserpin (NSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-105	96
  EDTA plasma(n=5)	88-95	91
  heparin plasma(n=5)	92-105	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroserpin (NSP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroserpin (NSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SERPINI1 Microplate: 96 Wells (12 x 8 Well Strips)
  • SERPINI1 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated SERPINI1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.34 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuroserpin (NSP). No significant cross-reactivity or interference between Neuroserpin (NSP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SERPINI1
• Gene Full Name: serpin family I member 1
• Alias Symbols: HNS-S1;HNS-S2;neuroserpin;peptidase inhibitor 12;PI12;PI-12;serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1;serpin I1;serpin peptidase inhibitor clade I member 1;serpin peptidase inhibitor, clade I (neuroserpin), member 1;truncated HNS-T.
• NCBI Gene Id: 5274
• Protein Name: Neuroserpin
• Description of Target: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin (PubMed:9442076, PubMed:26329378, PubMed:19265707, PubMed:19285087, PubMed:11880376). May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator (Probable).
• Uniprot ID: Q99574
• Protein Accession #: NP_001116224
• Nucleotide Accession #: NM_001122752.1
• Protein Size (# AA): 410