Catalog No: OKCD00387 (Formerly GWB-KBBCL5)
Size:96WELLS
Price: $675.00
SKU
OKCD00387
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Datasheets/ManualsPrintable datasheet for SERPINB9 ELISA Kit (Human) (OKCD00387)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)91-99%79-89%89-96%95-105%
EDTA plasma(n=5)95-103%80-89%78-94%88-99%
heparin plasma(n=5)78-103%94-105%83-95%99-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytoplasmic Antiproteinase 3 (CAP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytoplasmic Antiproteinase 3 (CAP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytoplasmic Antiproteinase 3 (CAP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytoplasmic Antiproteinase 3 (CAP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10298
EDTA plasma(n=5)98-105102
heparin plasma(n=5)94-10297
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytoplasmic Antiproteinase 3 (CAP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytoplasmic Antiproteinase 3 (CAP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SERPINB9 Microplate96 Wells (12 x 8 Well Strips)
SERPINB9 Lyophilized Standard2 x 10 ng
100X Biotinylated SERPINB9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytoplasmic Antiproteinase 3 (CAP3). No significant cross-reactivity or interference between Cytoplasmic Antiproteinase 3 (CAP3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSERPINB9
Gene Full Nameserpin family B member 9
Alias SymbolsCAP3;CAP-3;cytoplasmic antiproteinase 3;peptidase inhibitor 9;PI9;PI-9;protease inhibitor 9 (ovalbumin type);serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9;serpin B9;serpin peptidase inhibitor, clade B (ovalbumin), member 9;serpin peptidase inhibitor, clade B, member 9;testicular tissue protein Li 180.
NCBI Gene Id5272
Protein NameSerpin B9
Description of TargetGranzyme B inhibitor.
Uniprot IDP50453
Protein Accession #NP_004146
Nucleotide Accession #NM_004155.5
Protein Size (# AA)376
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